Super-Resolution Microscopy: A Virus’ Eye View of the Cell
نویسنده
چکیده
It is difficult to observe the molecular choreography between viruses and host cell components, as they exist on a spatial scale beyond the reach of conventional microscopy. However, novel super-resolution microscopy techniques have cast aside technical limitations to reveal a nanoscale view of virus replication and cell biology. This article provides an introduction to super-resolution imaging; in particular, localisation microscopy, and explores the application of such technologies to the study of viruses and tetraspanins, the topic of this special issue.
منابع مشابه
Particle tracking and inference in fluorescence microscopy
Observing biophysical phenomena at the nanometer scale with both high spatial and temporal resolution is a challenging feat. Although many techniques, including atomic force microscopy and scanning electron microscopy, have demonstrated subnanometer spatial resolution, most exhibit drawbacks which limit their temporal resolution. On the other hand, light microscopy exhibits poor spatial resolut...
متن کاملDeveloping the Adult Male ICRP Phantom and Evaluation the Absorbed Dose Received By Critical Organs in Head and Neck Region during the Radiotherapy of Eye Cancer
Introduction Accurate estimation of the absorbed dose in radiosensitive organs, located away from the target volume during radiotherapy, is one of the main reasons for the development of reference phantoms. The International Commission on Radiological Protection (ICRP) reference phantoms can provide a more realistic view of the human anatomy in comparison with the previously used mathematical p...
متن کاملQuantitative Multicolor Super-Resolution Microscopy Reveals Tetherin HIV-1 Interaction
Virus assembly and interaction with host-cell proteins occur at length scales below the diffraction limit of visible light. Novel super-resolution microscopy techniques achieve nanometer resolution of fluorescently labeled molecules. The cellular restriction factor tetherin (also known as CD317, BST-2 or HM1.24) inhibits the release of human immunodeficiency virus 1 (HIV-1) through direct incor...
متن کاملPALM and STORM: what hides beyond the Rayleigh limit?
Super-resolution imaging allows the imaging of fluorescently labeled probes at a resolution of just tens of nanometers, surpassing classic light microscopy by at least one order of magnitude. Recent advances such as the development of photo-switchable fluorophores, high-sensitivity microscopes and single particle localization algorithms make super-resolution imaging rapidly accessible to the wi...
متن کاملImproving Super-resolution Techniques via Employing Blurriness Information of the Image
Super-resolution (SR) is a technique that produces a high resolution (HR) image via employing a number of low resolution (LR) images from the same scene. One of the degradations that attenuates performance of the SR is the blurriness of the input LR images. In many previous works in the SR, the blurriness of the LR images is assumed to be due to the integral effect of the image sensor of the im...
متن کامل